Louise Laurent
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Assistant Professor Telephone: |
Education
2005-2008 UCSD. Fellowship in Maternal Fetal Medicine.
2001-2005 UCSD. Residency in Reproductive Medicine.
1990-2001 UCSF. Medical Scientist Training Program.
Ph.D. in Biochemistry awarded in 1998.
M.D. awarded in 2001.
1987-90 Harvard College. A.B. in Biochemistry.
1983-87 University of Chicago Laboratory High School.
Honors and Awards
2007 California Institute for Regenerative Medicine Junior Investigator Travel Award.
2006 California Institute for Regenerative Medicine Clinical Fellow Training Award
2004 Third Year Resident Research Award, University of California, San Diego.
1990 Summa Cum Laude and Harvard Hoopes Award for Outstanding Senior Thesis in Biochemistry.
1987 Westinghouse Science Talent Search. First Place Award.
1987 Presidential Scholar.
1987 Harvard National Scholar.
1986 Chicago Museum of Science and Industry's Outstanding Young Scientist.
Research Experience
April, 1983 - August, 1987. Student in Research. Cloning and characterization of preprocathepsin L and Egr-1. Howard Hughes Medical Institute at the University of Chicago. Supervised by Vikas P. Sukhatme.
June, 1988 - August, 1988. Student in Research. Characterization of EGR3, an addition to the EGR family of zinc finger proteins. Howard Hughes Medical Institute at the University of Chicago. Supervised by Vikas P. Sukhatme.
1989 - 1990. Undergraduate student. Site-directed mutagenesis of triosephosphate isomerase. Determination of kinetic parameters and crystallization of resulting mutants. Harvard University, Department of Chemistry. Supervised by Jeremy R. Knowles.
1990 - 1991. Medical student. Effect of the drosophila homologue of the myb proto-oncogene on transcription. UCSF, Department of Biochemistry. Supervised by J. Michael Bishop.
1991- 1993. Graduate student. Crystallization and diffraction of thymidylate synthetase with ligands. Biochemistry of HIV integrase. UCSF, Department of Biochemistry. Supervised by Harold E. Varmus and Robert M. Stroud.
1994 – 1998. Graduate student. Genetic footprinting of the HIV-1 genome. UCSF, Department of Biochemistry. Supervised by Patrick O. Brown.
2002 – 2005. Resident physician. Patterns of placental gene expression. UCSD, Department of Reproductive Medicine. Supervised by Dr. Thomas R. Moore.
2006 – 2008. Clinical Fellow. MicroRNA profiling of human embryonic stem cells, somatic stem cells, and differentiated cells. The Scripps Research Institute. Supervised by Jeanne Loring.
2008 – present. Assistant Professor. Integrative Genomics of human embryonic stem cells. UCSD and The Scripps Research Institute.
Hospital Appointments
2001-present University of California San Diego Medical Center
2005-present Scripps Mercy Hospital, San Diego, CA
2006-present Scripps Memorial Hospital, La Jolla, CA
2007-present Sharp Grossmont Hospital, San Diego, CA
2007-present Palomar Hospital, Escondido, CA
2007-present Pomerado Hospital, Poway, CA
Licensure and Certification
2002-present California Medical Board License #A80409
2002-present DEA License #BL8135623
2007 American Board of Obstetrics and Gynecology Board Certified
Professional Societies
2001-present American College of Obstetrics and Gynecology
2005-present Society for Maternal Fetal Medicine
2007-present Society for Gynecologic Investigation
2007-present International Society for Stem Cell Research
Research Support
8/01/2006-6/30/2007 California Institute for Regenerative Medicine Stem Cell Training Grant,
UCSD, Goldstein (PI).
Role: Clinical Fellow
7/01/2007-6/30/2008 Reproductive Scientist Development Program Scholar (NIH K12).
Role: Clinical Fellow
7/1/2008-present Women’s Reproductive Health Research Career Development Award (NIH K12).
Role: Assistant Professor
External Professional Activities
Laurent LC, Chen J, Mueller F-J, Williams R, Carneal B, Fan J-B, Loring J. 2007.
“miRNA regulation of human embryonic stem cells.” Oral presentation. Cold Spring Harbor Meeting on Systems Biology. Cold Spring Harbor, NY.
Laurent LC, Mueller F-J, Chen J, Carneal B, Williams R, Lu C, Fan J-B, Loring J. 2007.
“Coordinate regulation of miRNA clusters and families in human embryonic stem cells.” Oral Presentation. ISSCR. Cairns, Australia.
Laurent LC and Fan J-B. 2007. “miRNA regulation of human embryonic stem cells.”
Invited speaker at Discovery-2-Diagnostics conference. Philadelphia, PA.
Mueller F-J, Kostka D, Laurent LC, Ulitsky I, Lu C, Rao MS, Shamir R, Fan J-B, Bibikova
M, Loring JF. 2008. “Essential Pluripotence: Identification of common core networks defining pluripotent cells.” Oral presentation. Cold Spring Harbor Meeting on Systems Biology. Cold Spring Harbor, NY.
Laurent LC. 2008. “One of these things is not like the other one… or is it?” Invited oral
presentation. Are All Pluripotent Stem Cells Created Equal? Symposium at the Burnham Institute for Medical Research. La Jolla, CA.
Laurent LC. 2008. “Epigenetic Crossroads in Embryonic Stem Cells.” Invited oral presentation.
7th Annual Mount Desert island Stem Cell Symposium. Salisbury Cove, Maine.
Laurent LC. 2008. “Integrative Genomic and Epigenomic Analysis of Embryonic Stem Cells.”
Invited oral presentation. Illumina Seminar Series, From Whole-Genome to Whole-Solution, Disease Analysis Tools for the Next Generation. Scripps Translational Science Institute, La Jolla, California.
Laurent LC. 2008. “Genomic Approaches to Human Embryonic Stem Cells.” Seminar series.
Children’s Hospital Oakland Research Institute, Oakland, California.
Laurent LC. 2008. “The Epigenome of Human Embryonic Stem Cells.” Epigenie Seminar Series. La Jolla, California.
Reviewed manuscripts:
2007: Cell Stem Cells
2008: Journal of Alzheimer’s Disease, Cloning and Stem Cells, Journal of Cellular and Molecular Medicine, Nature Genetics
Reviewed grants:
2008: Wellcome Trust
Publications
Peer Reviewed Research Articles
Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP. 1988. "Complete nucleotide and
deduced amino acid sequences of human and murine preprocathepsin L, an abundant transcript induced by transformation of fibroblasts." Journal of Clinical Investigation. 81:1621-1629.
Sukhatme VP, Cao X, Chang LC, Tsai-Morris C-H, Stamenkovich D, Ferreira PCP, Cohen DR, Edwards SA, Shows TB, Curran T, Le Beau MM, Adamson ED. 1988. "A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization." Cell. 53:37-43.
Komives EA, Chang LC, Lolis E, Tilton RF, Petsko GA, Knowles JR. 1991. "Electrophilic catalysis in triosephosphate isomerase: the role of histidine-95." Biochemistry. 30(12):3011-9.
Patwardhan S, Gashler A, Siegel MG, Chang LC, Joseph LJ, Shows TB, Le Beau MM, Sukhatme VP. 1991. "EGR3, a novel member of the Egr family of genes encoding immediate early transcription factors." Oncogene. 6(6):917-28.
Perry KM, Carreras CW, Chang LC, Santi DV, Stroud RM. 1993. "Structures of thymidylate synthase with a C-terminal deletion: role of the C-terminus in alignment of 2'-deoxyuridine 5'- monophosphate and 5,10-methylenetetrahydrofolate." Biochemistry. 32(28):7116-25.
Lodi PJ; Chang LC; Knowles JR; Komives EA. 1994. "Triosephosphate isomerase requires a positively charged active site: the role of lysine-12." Biochemistry. 33(10):2809-14.
Laurent LC, Olsen MN, Crowley RA, Savilahti H, Brown PO. 2000. “Functional Characterization of the HIV-1 Genome by Genetic Footprinting.” Journal of Virology, 74(6):2760-2769.
Rothenberg SM, Olsen MN, Laurent LC, Crowley RA, Brown PO. 2001. “Comprehensive mutational analysis of the Moloney murine leukemia virus envelope protein.” Journal of Virology , 75(23):11851-62.
Laurent LC, Chen J, Ulitsky I, Mueller F-J, Lu C, Shamir R, Fan J-B, Loring JF. 2008.
" Comprehensive MicroRNA profiling reveals a unique human embryonic stem cell
signature dominated by a single seed sequence.” Stem Cells, 26:1506-1516.
Mueller F-J, Laurent LC, Kostka D, Ulitsky I, Williams R, Lu C, Rao MS, Shamir R, Schwartz PH,
Schmidt NO, Loring JF. 2008. “Regulatory networks define phenotypic classes of human stem cell lines.” Nature. 455:401-405.
Ulitsky I, Laurent LC, Mueller F-J, Loring JF, Shamir R. “Placing stem cell microRNAs in a network
context: Integrated analysis of microRNA expression and protein interactions.” in review.
Laurent LC, Ulitsky I, Nievergelt C, Park H-S, Graichen R, Murray S, Schork N, Shamir R, Loring
JF. Recurrent duplication of NANOG in human embryonic stem cells. manuscript in preparation.
Invited Articles and Reviews
Bibikova M, Laurent LC, Ren B, Loring JF, Fan J-B. 2008. “Unraveling the epigenome of embryonic stem cells.” Cell Stem Cell, 2(2):123-34.
Laurent LC. 2008. "MicroRNAs in Embryonic Stem Cells and Early Embryonic Development.” Journal of Cellular and Molecular Medicine, in press.
Books and Book Chapters
Rajan P, Smotrich D, Ross R, Laurent LC, Loring JF. 2007. “Derivation of Embryonic Stem Cells from Human Blastocysts.” In Loring JF, Wesselschmidt RL, and Schwartz PH, Human Stem Cell Manual, pp. 291-308, Elsevier.
Abstracts
Laurent LC. 1999. “Functional Characterization of the HIV-1 Genome by Genetic Footprinting.” Ph.D. dissertation. University of California at San Francisco.
Laurent LC and Moore TR. 2005. “Patterns of placental gene expression.” Poster.
Society for Maternal Fetal Medicine. Reno, NV.
Laurent LC, Smotrich D, Ross R, Williams R, Loring J. 2006. “Analysis of gene regulation
in the earliest stages of human embryonic development.” Poster. Genomes, Medicine, and the Environment Conference. The Venter Institute. Hilton Head, SC.
Mueller F-J, Williams R, Laurent LC, Kostka D, Ulitsky I, Shamir R, Schmidt NO, Loring J.
2007. “Stem Cell Matrix Database: Classification of Hundreds of Stem Cell Preparations using Transcriptional Networks.” Poster. Cold Spring Harbor Meeting on Systems Biology. Cold Spring Harbor, NY.
Fan J-B, Chen J, Laurent LC, Loring J, Barker D. 2007. “MicroRNA expression profiling in
cancer tissues using universal bead arrays.” Poster. American Association for Cancer Research Annual Meeting. Los Angeles, CA.
Laurent LC, Ross R, Williams R, Lu C, Chen C, Loring J. 2007. “mRNA and miRNA
profiling of intact and microdissected human blastocysts.” Poster. International Society for Stem Cell Research (ISSCR) Annual Meeting. Cairns, Australia.
Laurent LC, Chen J, Mueller F-J, Williams R, Carneal B, Fan, J-B, Loring J. 2007.
“Integration of genome-wide mRNA and miRNA profiling to identify regulatory pathways critical for self-renewal, pluripotency, and differentiation of human embryonic stem cells.” Poster. ISSCR. Cairns, Australia.
Fan J-B, Chen J, Loring J, Laurent LC. 2007. “MicroRNA expression profiling in human
embryonic stem cells using universal bead arrays.” Poster. American Society of Human Genetics Annual Meeting. San Diego, CA.
Laurent LC, Frazer K, Murray S, Loring JF. 2008. “Alterations in gene expression
corresponding to recurrent genetic variations observed in human embryonic stem cell cultures.” Poster. Cold Spring Harbor Meeting on Systems Biology. Cold Spring Harbor, NY.
Laurent LC, Bibikova M, Jaeger E, Lu C, Chen J, Loring JF, Fan J-B. 2008. “Evidence for DNA
hypermethylation as a mechanism for repression of microRNAs expression in human embryonic stem cells.” Poster. ISSCR. Philadelphia, PA.
Laurent LC, Nievergelt C, Frazer K, Ulitsky I, Murray S, Shamir R, Loring JF, Schork, N. 2008.
“SNP Genotyping as a tool to assess ancestry and genetic stability of human embryonic stem cells.” Poster. ISSCR. Philadelphia, PA.
Albert K, Sheridan S, Laurent L, Loring J, Rao R. 2008. “Propagation of Human Embryonic
Stem Cells in an Indirect Microporous Membrane-Based Co-Culture System.” Poster. ISSCR. Philadelphia, PA.
Ben-Nun IF, Lynch C, Lieu P, Fontes A, Chesnut J, Laurent LC, Loring JF. 2008. “Cellular
reprogramming by microRNAs.” Poster. Stem Cells on the Mesa. San Diego, CA.
Garitaonandia I, Ulitsky I, Murray S, Loring JF, Laurent LC. 2008. “SNP Genotyping Analysis
as a Tool to Asses Genetic Stability of Human Embryonic Stem Cells.” Poster. Stem Cells on the Mesa. San Diego, CA.
Altun G, Loring JF, Laurent LC. 2008. “Classification and Analysis of Human Stem Cell Lines.”
Poster. Stem Cells on the Mesa. San Diego, CA.
Tache V, Laurent LC, Parast M. 2008. “The role of hypoxia in regulation of trophoblast
proliferation and gene expression.” Poster. Society for Maternal Fetal Medicine. San Diego, CA.